loading control protein α tubulin  (Cell Signaling Technology Inc)

Journal: BMC Biochemistry

Article Title: Chemical-genetic induction of Malonyl-CoA decarboxylase in skeletal muscle

doi: 10.1186/1471-2091-15-20

Figure Lengend Snippet: MCD transgene expression and Shield-1 stabilization is specific to skeletal muscle. Western blot of (A) liver (B) pancreas and (C) heart tissue extracted from Tg-fMCD skel and control mice on HFD and LFD treated with Shield-1 (5 doses at 60 mg/kg) or vehicle. Samples were blotted for FKBP12 (for transgene stabilization), MCD (endogenous expression), mCherry (transgene expression), and alpha Tubulin (loading control). Positive controls for mCherry and FKBP are derived from skeletal muscle of Tg-fMCD skel mice treated with vehicle or Shield-1.

Article Snippet: Alpha-Tubulin protein loading control was obtained from Sigma.

Techniques: Expressing, Western Blot, Derivative Assay

Journal: BMC Biochemistry

Article Title: Chemical-genetic induction of Malonyl-CoA decarboxylase in skeletal muscle

doi: 10.1186/1471-2091-15-20

Figure Lengend Snippet: MCD induction suppresses the fatty acid oxidative pathway. Western blot of gastrocnemius muscle for isolated from Tg-fMCD skel and control mice on LFD or HFD given Shield-1 (60 mg/kg) or vehicle for 5 days. (A) CPT1B and OXPHOS complexes (as indicated) were normalized to alpha Tubulin and quantified. Samples were blotted with FKBP12 (for transgene stabilization), mCherry (transgene expression) and alpha Tubulin (loading control). (B) MCAD and (C) HADHA were normalized to alpha Tubulin and quantified (D) AMPK Thr 172 phophorylation was determined, normalized for total AMPK and quantified. Data are expressed as means +/- SEM. *p < 0.05 by one-way ANOVA.

Article Snippet: Alpha-Tubulin protein loading control was obtained from Sigma.

Techniques: Western Blot, Isolation, Expressing